The HiSeq 2000/2500 System is a powerful and efficient ultra-high-throughput sequencing system that supports the broadest range of applications and study sizes. Unrivaled data quality using Illumina's proven SBS chemistry has made the HiSeq 2000/2500 the instrument of choice for all major genome centers and leading institutions throughout the world.
• HiSeq 2000/2500 offers unprecedented flexibility for multiple applications
The HiSeq 2500 System features two run modes, rapid run and high output run mode, and the ability to process one or two flow cells simultaneously. This provides a flexible and scalable platform that supports the broadest range of applications and study sizes. Choose between rapid run and high output modes to fit your project needs. New high output reagents enable the generation of up to 1 terabase (Tb) of data, supporting the greatest number of samples per run while the new rapid run reagents enable reads up to 2x250 base pairs.
• Rapid run mode provides quick results
Rapid run mode provides quick results, allows efficient processing of a limited number of samples, and offers support of longer paired-end 150 base pair reads. The longer reads enable greater depth of coverage, improved assembly for de novo applications and aid in long read applications such as metagenomic analyses.
• High output mode is perfect for larger studies
High output mode is ideal for larger studies or when the greatest depth of coverage is required. High output mode allows you to batch process > 6× more samples than rapid mode and to complete large projects quickly and efficiently. New HiSeq SBS Kits v4 produce 4 billion clusters per run, generating up to 1 Tb of data in 6 days.
| Targeted Sequencing | Choose from a set of targeted re-sequencing programs from a single custom panel to the entire exome, to support all research design. Cost-effective and scalable exploration, validation and screening is now at your fingertips. |
| Whole genome DNA sequencing |
From the high-throughput gene expression profiling transcription depth analysis, HiSeq provides unprecedented RNA analysis program. Determination of single transcript abundance and subtypes, the discovery of new genes and to identify non-coding regulatory RNA. |
| Epigenetic regulation | In HiSeq single run, by evaluating various forms of epigenetic regulation, a more complete understanding of the biological story. Utilization of DNA and protein interaction and gene regulation ChIP-Seq. In single-base resolution quantitative DNA methylation. |